Use of human tissue stem cell-derived organoid cultures to model enterohepatic circulation
Sarah E. Blutt, Sue E. Crawford, Carolyn Bomidi, Xi‐Lei Zeng, James R. Broughman, Matthew J. Robertson, Cristian Coarfa, Mary Elizabeth M. Tessier, Tor Savidge, F. Blaine Hollinger, Steven A. Curley, Mark Donowitz, Mary K. Estes
2021American Journal of Physiology-Gastrointestinal and Liver Physiology16 citationsDOIOpen Access PDF
Abstract
Interorgan signaling is a critical feature of human biology and physiology, yet has remained difficult to study due to the lack of in vitro models. Here, we demonstrate that physical coupling of ex vivo human intestine and liver epithelial organoid cultures recapitulates in vivo interorgan bile acid signaling. These results suggest that coupling of multiple organoid systems provides new models to investigate interorgan communication and advances our knowledge of human physiological and pathophysiological processes.
Topics & Concepts
OrganoidBiologyEnterohepatic circulationStem cellCell biologyIn vivoEx vivoBile acidEndocrinologyGeneticsLiver physiology and pathologyPancreatic and Hepatic Oncology ResearchCancer Cells and Metastasis