Strategic design of an NIR probe for viscosity imaging in inflammatory and non-alcoholic steatohepatitis mice
Yaogeng Ma, Min Li, Huilin Sun, Jing‐Yuan Ge, Bai Yang, Lin Qiu, Xuan Wu, Jiuxi Chen, Zhongyan Chen
Abstract
Two novel near-infrared (NIR) fluorescent probes Cy-Vis1 and Cy-Vis2 with large Stokes shifts (>100 nm) were constructed using a "symmetry collapse" strategy. Notably, Cy-Vis2 was significantly more sensitive to viscosity than Cy-Vis1 through an enhanced intramolecular interaction strategy. The fluorescence intensities of Cy-Vis1 and Cy-Vis2 exhibited increases, by 7.6- and 19.9-fold, respectively, across the viscosity range from 0.8 cp to 359.9 cp. Cy-Vis2 was successfully used to visualize viscosity abnormalities in lipopolysaccharide (LPS)-induced inflammatory and NASH model mice.
Topics & Concepts
SteatohepatitisViscosityChemistryMaterials scienceNanotechnologyBiomedical engineeringMedicinePathologyFatty liverComposite materialDiseaseLiver Disease Diagnosis and TreatmentSulfur Compounds in BiologySphingolipid Metabolism and Signaling