Photoswitchable inhibitors of human β-glucocerebrosidase
Maria Giulia Davighi, Francesca Clemente, Camilla Matassini, Francesca Cardona, Mogens Brøndsted Nielsen, Andrea Goti, Amelia Morrone, Paolo Paoli, Martina Cacciarini
Abstract
Light-switchable inhibitors of the enzyme β-glucocerebrosidase (GCase) have been developed by anchoring a specific azasugar to a dihydroazulene or an azobenzene responsive moiety. Their inhibitory effect towards human GCase, before and after irradiation are reported, and the effect on thermal denaturation of recombinant GCase and cytotoxicity were studied on selected candidates.
Topics & Concepts
GlucocerebrosidaseChemistryPharmacologyEnzymeBiochemistryMedicinePhotochromic and Fluorescence ChemistryPhotoreceptor and optogenetics researchBiochemical Analysis and Sensing Techniques