Charge detection mass spectrometry for the analysis of viruses and virus-like particles
Lohra M. Miller, Martin F. Jarrold
Abstract
Heterogeneity usually restricts conventional mass spectrometry to molecular weights less than around a megadalton. As a single-particle technique, charge detection mass spectrometry (CDMS) overcomes this limitation. In CDMS, the mass-to-charge (m/z) ratio and charge are measured simultaneously for individual ions, giving a direct mass measurement for each ion. Recent applications include the analysis of viruses, virus-like particles, vaccines, heavily glycosylated proteins, and gene therapy vectors.
Topics & Concepts
Mass spectrometryCharge (physics)IonChemistryAnalytical Chemistry (journal)Particle (ecology)ChromatographyPhysicsBiologyParticle physicsEcologyOrganic chemistryMass Spectrometry Techniques and ApplicationsBacteriophages and microbial interactionsRNA and protein synthesis mechanisms