FAMSi: A Synthetic Biology Approach to the Fast Assembly of Multiplex siRNAs for Silencing Gene Expression in Mammalian Cells
Fang He, Na Ni, Zongyue Zeng, Di Wu, Yixiao Feng, Alexander J. Li, Benjamin Luu, Alissa Li, Kevin Qin, Eric Wang, Xi Wang, Xiaoxing Wu, Huaxiu Luo, Jing Zhang, Meng Zhang, Yukun Mao, Mikhail Pakvasa, William Wagstaff, Yongtao Zhang, Changchun Niu, Hao Wang, Linjuan Huang, Deyao Shi, Qing Liu, Xia Zhao, Kai Fu, Russell R. Reid, Jennifer Moriatis Wolf, Michael J. Lee, Kelly Hynes, Jason Strelzow, Mostafa H. El Dafrawy, Hua Gan, Tong‐Chuan He, Jiaming Fan
Abstract
ectopic bone formation. Collectively, we demonstrate that the engineered FAMSi system provides a fast-track platform for assembling multiplexed siRNAs in a single vector, and thus it may be a valuable tool to study gene functions or to develop novel siRNA-based therapeutics.
Topics & Concepts
RNA interferenceSmall interfering RNAGene silencingTrans-acting siRNABiologyCell biologyRNA silencingGene expressionRNAGeneComputational biologyMolecular biologyGeneticsRNA Interference and Gene DeliveryAdvanced biosensing and bioanalysis techniquesCRISPR and Genetic Engineering