Autophagy core protein ATG5 is required for elongating spermatid development, sperm individualization and normal fertility in male mice
Qian Huang, Yunhao Liu, Shiyang Zhang, Yi Tian Yap, Wei Li, David Zhang, Ahmad Gardner, Ling Zhang, Shizheng Song, Rex A. Hess, Zhibing Zhang
Abstract
: AKAP4: a-kinase anchoring protein 4; ATG5: autophagy-related 5; ATG7: autophagy-related 7; ATG10: autophagy-related 10; ATG12: autophagy-related 12; cKO: conditional knockout; DDX4: DEAD-box helicase 4; MAP1LC3/LC3/tg8: microtubule-associated protein 1 light chain 3; PBS: phosphate-buffered saline; PIWIL2/MILI: piwi like RNA-mediated gene silencing 2; RT-PCR: reverse transcription-polymerase chain reaction; SQSTM1/p62: sequestosome 1; TBC: tubulobulbar complexes; WT: wild type.
Topics & Concepts
BiologyATG5SpermatidAutophagyAcrosomeSpermatogenesisSpermCell biologyAndrologySpermiogenesisGerm cellEndocrinologyApoptosisGeneticsMedicineGeneAutophagy in Disease and TherapySperm and Testicular FunctionEpigenetics and DNA Methylation