Amphiphilic Chitosan Bearing Double Palmitoyl Chains and Quaternary Ammonium Moieties as a Nanocarrier for Plasmid DNA
Thev Pol, Wunpen Chonkaew, Lalintip Hocharoen, Nakorn Niamnont, Namphueng Butkhot, Yaowaluck Maprang Roshorm, Suda Kiatkamjornwong, Voravee P. Hoven, Kornkanya Pratumyot
Abstract
) of 265.5 ± 7.40 nm (PDI = 0.5) and a surface charge potential of 40.1 ± 0.04 mV. bPalm-CS-HTAP condensed the plasmid pVAX1.CoV2RBDme completely at a bPalm-CS-HTAP:pDNA ratio of 2:1. The self-assembled bPalm-CS-HTAP/pDNA complexes could enter HEK 293A and CHO cells and enabled gene expression at negligible cytotoxicity compared to commercial PEI (20 kDa). These results suggested that bPalm-CS-HTAP can be used as a promising nonviral gene carrier.
Topics & Concepts
AmphiphileChemistryNanocarriersPlasmidAmmoniumChitosanDNABiochemistryOrganic chemistryDrug deliveryCopolymerPolymerRNA Interference and Gene DeliveryAdvanced biosensing and bioanalysis techniquesBacteriophages and microbial interactions