High resolution profiling of cell cycle-dependent protein and phosphorylation abundance changes in non-transformed cells
Camilla Rega, Ifigenia Tsitsa, Theodoros I. Roumeliotis, Izabella Krystkowiak, María Portillo, Lu Yu, Julia Vorhauser, Jonathon Pines, Jörg Mansfeld, Jyoti S. Choudhary, Norman E. Davey
Abstract
The cell cycle governs a precise series of molecular events, regulated by coordinated changes in protein and phosphorylation abundance, that culminates in the generation of two daughter cells. Here, we present a proteomic and phosphoproteomic analysis of the human cell cycle in hTERT-RPE-1 cells using deep quantitative mass spectrometry by isobaric labelling. By analysing non-transformed cells and improving the temporal resolution and coverage of key cell cycle regulators, we present a dataset of cell cycle-dependent protein and phosphorylation site oscillation that offers a foundational reference for investigating cell cycle regulation. These data reveal regulatory intricacies including proteins and phosphorylation sites exhibiting cell cycle-dependent oscillation, and proteins targeted for degradation during mitotic exit. Integrated with complementary resources, our data link cycle-dependent abundance dynamics to functional changes and are accessible through the Cell Cycle database (CCdb), an interactive web-based resource for the cell cycle community. The cell cycle governs a precise series of molecular events, regulated by coordinated changes in protein and phosphorylation abundance, that culminates in the generation of two daughter cells. Here, the authors present a proteomic and phosphoproteomic analysis of the human cell cycle in nontransformed cells using deep quantitative mass spectrometry by isobaric labelling.