Norepinephrine changes behavioral state through astroglial purinergic signaling
Alex Chen, Marc Duque, Altyn Rymbek, Mahalakshmi Dhanasekar, Vickie M. Wang, Xuelong Mi, Loeva Tocquer, Sujatha Narayan, Emmanuel Márquez-Legorreta, Mark Eddison, Guoqiang Yu, Claire Wyart, David A. Prober, Florian Engert, Misha B. Ahrens
Abstract
Both neurons and glia communicate through diffusible neuromodulators; however, how neuron-glial interactions in such neuromodulatory networks influence circuit computation and behavior is unclear. During futility-induced behavioral transitions in the larval zebrafish, the neuromodulator norepinephrine (NE) drives fast excitation and delayed inhibition of behavior and circuit activity. We found that astroglial purinergic signaling implements the inhibitory arm of this motif. In larval zebrafish, NE triggers astroglial release of adenosine triphosphate (ATP), extracellular conversion of ATP into adenosine, and behavioral suppression through activation of hindbrain neuronal adenosine receptors. Our results suggest a computational and behavioral role for an evolutionarily conserved astroglial purinergic signaling axis in NE-mediated behavioral and brain state transitions and position astroglia as important effectors in neuromodulatory signaling.