A red-emitting mitochondria targetable fluorescent probe for detecting viscosity in HeLa, zebrafish, and mice
Xi Gong, Rui Guo, X. D. Li, Yingjie Yang, Weiying Lin
Abstract
. The probe GX-VS had many advantages such as long emission wavelength (650 nm), large Stokes shift (105 nm), significant fluorescence enhancement (59-fold), high sensitivity, good biocompatibility and so on. Biological experiments showed that the probe could target mitochondria and detect viscosity alterations in HeLa cells. Moreover, it has been successfully utilized to monitor viscosity changes induced by lipopolysaccharides (LPS) in inflammatory zebrafishes and living mice, which further underscored the capacity of GX-VS to explore fluctuations in viscosity within living organisms.
Topics & Concepts
HeLaFluorescenceZebrafishViscosityIn vivoBiocompatibilityBiophysicsMitochondrionChemistryIn vitroStokes shiftCell biologyMaterials scienceBiologyBiochemistryBiotechnologyPhysicsGeneQuantum mechanicsComposite materialOrganic chemistryMolecular Sensors and Ion DetectionLuminescence and Fluorescent MaterialsNitric Oxide and Endothelin Effects