Reduced SLIT2 is Associated with Increased Cell Proliferation and Arsenic Trioxide Resistance in Acute Promyelocytic Leukemia
Isabel Weinhäuser, Diego A. Pereira‐Martins, César Alexander Ortiz Rojas, Douglas RA Silveira, Luíse A A Simões, Thiago Mantello Bianco, Cleide L. Araujo, Luisa C A Koury, Raul Antônio Morais Melo, Rosane Bittencourt, Kátia Bórgia Barbosa Pagnano, Ricardo Pasqüini, Elenaide C. Nunes, Evandro M. Fagundes, Ana Glória, Fábio R. Kerbauy, Maria de Lourdes Chauffaille, Armand Keating, Martin S. Tallman, Raul C. Ribeiro, Richard Dillon, Arnold Ganser, Bob Löwenberg, Peter J.M. Valk, Francesco Lo‐Coco, Miguel Á. Sanz, Nancy Berliner, Emanuele Ammatuna, Antonio R. Lucena‐Araujo, Jan Jacob Schuringa, Eduardo Magalhães Rego
Abstract
The SLIT-ROBO axis plays an important role in normal stem-cell biology, with possible repercussions on cancer stem cell emergence. Although the Promyelocytic Leukemia (PML) protein can regulate SLIT2 expression in the central nervous system, little is known about SLIT2 in acute promyelocytic leukemia. Hence, we aimed to investigate the levels of SLIT2 in acute promyelocytic leukemia (APL) and assess its biological activity in vitro and in vivo. Our analysis indicated that blasts with SLIT2high transcript levels were associated with cell cycle arrest, while SLIT2low APL blasts displayed a more stem-cell like phenotype. In a retrospective analysis using a cohort of patients treated with all-trans retinoic acid (ATRA) and anthracyclines, high SLIT2 expression was correlated with reduced leukocyte count (p = 0.024), and independently associated with improved overall survival (hazard ratio: 0.94; 95% confidence interval: 0.92–0.97; p < 0.001). Functionally, SLIT2-knockdown in primary APL blasts and cell lines led to increased cell proliferation and resistance to arsenic trioxide induced apoptosis. Finally, in vivo transplant of Slit2-silenced primary APL blasts promoted increased leukocyte count (p = 0.001) and decreased overall survival (p = 0.002) compared with the control. In summary, our data highlight the tumor suppressive function of SLIT2 in APL and its deteriorating effects on disease progression when downregulated.