Molecular vibrational imaging by stimulated Raman scattering microscopy: principles and applications [Invited]
Yasuyuki Ozeki
Abstract
The recent development of stimulated Raman scattering (SRS) microscopy allows for highly sensitive biological imaging with molecular vibrational contrast, opening up a variety of applications including label-free imaging, metabolic imaging, and super-multiplex imaging. This paper introduces the principle of SRS microscopy and the methods of multicolor SRS imaging and describes an overview of biomedical applications.
Topics & Concepts
MicroscopyRaman scatteringMolecular imagingRaman spectroscopyOpticsMultiplexMaterials scienceBiological imagingMedical imagingNanotechnologyComputer sciencePhysicsFluorescenceBioinformaticsArtificial intelligenceBiologyIn vivoBiotechnologySpectroscopy Techniques in Biomedical and Chemical ResearchPhotoacoustic and Ultrasonic ImagingNanoplatforms for cancer theranostics