FT-IR, GC-MS, and HPLC Profiling of the Bioactive Constituents of Ethyl Acetate Fraction of Eichhornia crassipes as a Hepatoprotectant
Anuradha Shukla, Saya Tyagi, Varsha Gupta, Pankaj Jain, Tapan Kanai, Rashmi Tripathi, R Rouf, P Ghosh, Md Uzzaman, D Sarker, F Zahura, S Uddin, I Muhammad, F Guo, L Feng, C Huang, H Ding, X Zhang, Z Wang, Y Li, M Farid, S Hussein, L Ibrahim, M El Desouky, A Elsayed, A El Oqlah, M Saker, L Krepkova, A Babenko, ' Saybel, O Lupanova, I Kuzina, O Job, K Sherwin, C Enioutina, E, C.-C Chang, W Cheng, G.-C Yen, H.-Y Chen, H Ali, M Patel, N Ganesh, J Ahi, M Pakkirisamy, S Kalakandan, K Ravichandran, M Poojitha, S Kumar, S Narayana, M Jeevitha, P Ravi, V Subramaniyam, M Pichumani, S Sripathi, W Ahmed, R Azmat, A Mehmood, A Qayyum, R Ahmed, S Khan, M Liaquat, S Naz, S Ahmad, M Thenmozhi, M Sangeetha, M Jayanthi, M Suganthi, R Ramya, L Ralte, L Khiangte, N Thangjam, A Kumar, Y Singh, N Konappa, A Udayashankar, S Krishnamurthy, C Pradeep, S Chowdappa, S Jogaiah, K Shalini, K Ilango, K Shalini, K Ilango, J Khan, A Majid, N Nazir, M Nisar, A Khan Khalil, M Zahoor, M Ihsan, R Ullah, A Bari, A Shah, S Ganguly, J Kumar, T Seal, D Kumar, M Karthik, R Rajakumar
Abstract
The study was carried out to analyze the phytoconstituents of the leaves of the aquatic weed E. crassipes.The plant is being traditionally used as a medicinal plant due to its bioactive constituents, but not many reports are available about E.crassipes as a hepatoprotection. The bioactive substances in the ethyl acetate fraction of the hydroethanolic extract of the leaves of E. crassipes (Mart.) Solms were analyzed by using Fourier Transform Infrared Spectroscopy (FT-IR), Gas Chromatography-Mass Spectrometer (GC-MS), and High-Pressure Liquid Chromatography (HPLC). The IR spectrum of ethyl acetate fraction of the hydroethanolic extract shows 16 peaks corresponding to peaks ranging from wave number 3624.42 cm-1 to 610.40 cm-1. In the GC-MS analysis the existence of 3,5-bis(1,1-dimethylethyl)- (C8H12O3), 7 oxabicyclo [4.1.0] heptan-1-ol, acetate(C14H22O), 7,9-di-tert-butyl-1-oxaspiro (4.5) deca-6,9-diene-2,8-dione, palmitic acid vinyl ester (C18H34O2), phytol (C20H40O) as the major constituent of the extract. The extract was further analyzed by HPLC, indicating the presence of phytol as a major constituent that plays a vital role in hepatoprotection. Thus from the results, it is evident that it contains various bioactive compounds and can recommend as a plant of pharmaceutical importance.