Diagnostic Yield and Impact on Antimicrobial Management of 16S rRNA Testing of Clinical Specimens
Pruke Eamsakulrat, Pitak Santanirand, Angsana Phuphuakrat
Abstract
16S rRNA gene sequencing has been increasingly used in clinical practice. Using the final diagnosis as a gold standard, the sensitivity of 16S rRNA gene sequencing was fair. In the setting with no 16S rRNA gene sequencing test ordering restrictions, only small percentages of the test results had an impact on antimicrobial management. Restrictions on test requests should be developed to maximize the benefit of the test.
Topics & Concepts
16S ribosomal RNAMicrobiological cultureGold standard (test)Ribosomal RNABiologyAntimicrobialAntibioticsClinical microbiologyMedicineMicrobiologyInternal medicineGeneBacteriaGeneticsInfective Endocarditis Diagnosis and ManagementBacterial Identification and Susceptibility TestingAntimicrobial Resistance in Staphylococcus