Osteoclast-derived small extracellular vesicles induce osteogenic differentiation via inhibiting ARHGAP1
Mengmeng Liang, Xiaofan Yin, Shuai Zhang, Hongbo Ai, Fei Luo, Jianzhong Xu, Ce Dou, Shiwu Dong, Qinyu Ma
Abstract
, a negative regulator of osteogenic differentiation. We next fabricated an sEV-modified scaffold by coating decalcified bone matrix (DBM) with osteoclast-derived sEVs, and the pro-osteogenic regeneration activities of the sEV-modified scaffold were validated in a mouse calvarial defect model. Notably, miR-324-enriched sEV-modified scaffold showed the highest capacity on bone regeneration, whereas inhibition of miR-324 in sEVs abrogated these effects. Taken together, our findings suggest that miR-324-contained sEVs released from mature osteoclast play an essential role in the regulation of osteogenic differentiation and potentially bridge the coupling between osteoclasts and MSCs.