Phytochemical mediated synthesis of silver nanoparticles and their antibacterial activity
Lakshman Kumar Dogiparthi, Siva Sankar Sana, Sumaya Zikreen Shaik, Maneesha Reddy Kalvapalli, Gayathri Kurupati, Godlaveeti Sreenivasa Kumar, Lekshmi Gangadhar
Abstract
Abstract In this present work, we described a bio-reduction method for the generation of silver nanoparticles (AgNPs) using aqueous leaf extract of Micrargeria wightii ( M. wightii ), which is a gifted alternative to other physicochemical routes. The prepared AgNPs were characterized by UV–visible spectroscopy (UV–vis), Fourier Transform Infrared Spectroscopy (FT-IR), X-ray diffraction (X-RD), Transmission Electron Microscopy (TEM) with EDX and Dynamic light scattering (DLS). UV–visible spectrum showed a characteristic absorption peak at 440 nm of synthesized AgNPs. FT-IR analysis confirmed the existence of plant metabolites, which are responsible for the reduction of Ag (I) ions into Ag (0) NPs. X-RD pattern studies confirm the presence of the pure face-centered cubiccrystalline nature of Ag. Energy-dispersive X-ray (E-DX) spectrum showed the elemental composition of synthesized nanoparticles. Furthermore, TEM images confirm the formation of spherical shaped nano-silver particles with sizes ranging from 30 to 70 nm and supported by particle size analyzer, Dynamic Light Scattering (DLS). Thus, the present investigation provides an easy, eco-friendly and straightforward route for the synthesis of the antibacterial agent against Bacillus subtilis subtilis and Pseudomonas aeruginosa , with 15 and 13 mm zone of inhibition (ZOI) respectively.