Spatial profiling technologies and applications for brain cancers
Priyakshi Kalita‐de Croft, Habib Sadeghi Rad, Harry Gasper, Kenneth J. O’Byrne, Sunil R. Lakhani, Arutha Kulasinghe
Abstract
INTRODUCTION: Malignant primary and secondary brain tumors pose a major health challenge, and the incidence of these tumors is rising. The brain tumor microenvironment (TME) is highly complex and thought to impact treatment resistance and failure. To enable a greater understanding of the milieu of cells in the brain TME, advances in imaging and sequential profiling of proteins/mRNA have given rise to the field of spatial transcriptomics. These technologies provide a greater depth of understanding of the tissue architecture, cellular and spatial profiles, including cellular activation status, which may provide insights into effective therapies for brain cancers. AREAS COVERED: In this review, we provide an overview of spatial profiling technologies at the forefront in the field and describe the applications for brain cancer. EXPERT OPINION: Brain tumors are often resistant to treatment, and display both an immunosuppressive and heterogeneous tumor microenvironment. Next-generation imaging and multi-omics technologies are providing a tool for intricately characterizing their tissue biology. This information will aid in the design of effective therapies and begin to provide an understanding of therapy resistance.