Targeted MYC2 stabilization confers citrus Huanglongbing resistance
Pingzhi Zhao, Huan Yang, Yanwei Sun, J. Q. Zhang, Kaixing Gao, Jinbao Wu, Chengrong Zhu, Cece Yin, Xiaoyue Chen, Qi Liu, Qiudong Xia, Qiong Li, Han Xiao, Hai‐Xi Sun, Xiaoxiao Zhang, Long Yi, Changyong Zhou, Daniel J. Kliebenstein, Rongxiang Fang, Xuefeng Wang, Jian Ye
Abstract
Huanglongbing (HLB) is a devastating citrus disease. In this work, we report an HLB resistance regulatory circuit in Citrus composed of an E3 ubiquitin ligase, PUB21, and its substrate, the MYC2 transcription factor, which regulates jasmonate-mediated defense responses. A helitron insertion in the PUB21 promoter introduced multiple MYC2-binding cis-elements to create a regulatory circuit linking the PUB21 activity with MYC2 degradation. Ectopic expression of a natural dominant-negative PUB21 paralog discovered in distant Citrus relatives stabilized MYC2 and conferred resistance to HLB. Antiproteolysis peptides (APPs), identified by artificial intelligence, stabilized MYC2 by binding and inhibiting PUB21 activity. A 14–amino acid peptide, APP3-14, molecularly controlled HLB in greenhouse and field trials. This approach represents a strategy to combat uncultivable pathogens through targeted disease resistance protein stabilization.