GENOME-WIDE BIOINFORMATICS ANALYSIS OF 1-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE (ACS), 1-AMINOCYCLOPROPANE-1-CARBOXYLATE OXIDASE (ACO) AND ETHYLENE OVERPRODUCER 1 (ETO1) GENE FAMILY OF FRAGARIA VESCA (WOODLAND STRAWBERRY)
S FATIMA, KUHT CHEEMA, Muhammad Shafiq, MT MANZOOR, Qurban Ali, MS HAIDER, Muhammad Umair Shahid
Abstract
This study was made on three genes ACS, ACO, and ETO, which are involved in ethylene biosynthesis pathway in Fragaria vesa plant, to know about evolution, conserved motifs and domains, gene expressions, and phylogeny of these genes. After carefully screening using Phytozome plant gene database, NCBI gene database, Motif finder, and MegaX phylogenetic tree 10 gene sequences of ACS, 5 gene sequences of ACO and 3 gene sequences of ETO were identified. Four ETO gene sequences of Arabidopsis thaliana were also used to authenticate this research because only 3 ETO gene sequences of Fragaria vesca analyses cannot be done. MegaX evolutionary analysis, TB tools domain analysis, Meme motif analysis, Cis-regulatory analysis, Wolf analysis were made on these sequences to acquire detailed knowledge. The presence of light, anaerobic induction, abscisic acid, MeJA, gibberellin, low temperature, drought, cell cycle, and endosperm expression responsive elements were identified in FeACS, FeACO, and FeETO genes by cis-regulatory analysis. This study will help for further practical experimentation on ethylene regulators. The bioinformatics-based genome-wide assessment of the family of Fragaria vesca attempted in the present study could be a significant step for further practical investigation on ethylene regulators based on genome-wide expression profiling.