High-throughput discovery of phage receptors using transposon insertion sequencing of bacteria
Kaitlyn E. Kortright, Benjamin K. Chan, Paul E. Turner
Abstract
(phages T6, T2, T4, and T7). INSeq screens were then used to identify genes involved during infection of six newly isolated coliphages. Results showed that candidate receptors could be successfully identified for the majority (five of six) of the phages; furthermore, genes encoding the phage receptor(s) were the top hit(s) in the analyses of the successful screens. INSeq screens provide a generally useful method for high-throughput discovery of phage receptors. We discuss limitations of our approach when examining uncharacterized phages, as well as usefulness of the method for exploring the evolution of broad versus narrow use of cellular receptors among phages in the biosphere.
Topics & Concepts
BiologyBacteriaTransposable elementReceptorBacteriophageBacterial virusMicrobiologyComputational biologyGenomeGeneticsGeneEscherichia coliBacteriophages and microbial interactionsMicrobial infections and disease researchMonoclonal and Polyclonal Antibodies Research