Xihuang pill induces pyroptosis and inhibits progression of breast cancer cells via activating the cAMP/PKA signalling pathway.
Cong Chen, Shanmin Yuan, Xiaohong Chen, Jiangping Xie, Wei Zheng
Abstract
by generating murine mammary carcinoma 4T1 cell xenografts. Enzyme-linked immunosorbent assay (ELISA) and reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) were used to detect pyroptosis-related indicators. Transcriptome sequencing was used to identify potential targets of XHP. Cell counting kit-8 (CCK-8), EdU, cell wound-healing, and transwell assays were performed to investigate the role of XHP in BC cells. Western blotting was performed to identify proteins related to the cAMP/PKA signalling pathway. XHP inhibited the growth of BC and induced pyroptosis. Western blotting confirmed the significant association between XHP and the cAMP/PKA signalling pathway revealed by Kyoto Encyclopedia of Genes and Genomes pathway analysis. XHP inhibited BC cell proliferation, migration, and invasion, and induced pyroptosis. Inhibiting the cAMP/PKA signalling pathway reversed the anti-cancer activity of XHP. XHP inhibits proliferation, migration, and invasion, and induces pyroptosis of BC cells via activating the cAMP/PKA signalling pathway.