Ozonated Aloe vera Oil Effective Increased the Number of Fibroblasts and Collagen Thickening in the Healing Response of Full-Thickness Skin Defects
Ahsanu Taqwim Hidayat, Muhamad Thohar Arifin, Muhammad Nur, Muflihatul Muniroh, Neni Susilaningsih
Abstract
Objective. This study aimed to examine the effectiveness of ozonated Aloe vera oil on the wound healing response of full-thickness defect tissue in Sprague-Dawley rats, assessed by collagen thickness and the number of fibroblasts. Methods. This was an experimental research method using control groups and treatment groups with a posttest only control group design. The results showed that collagen thickness in wounds tended to increase, assessed on day 3 and day 7 using Masson’s trichrome staining and microscopic evaluation. Results. There was a significant difference in the number of fibroblasts between the two control and treatment groups on days 3 and 7 tested using one-way Kruskal–Wallis test, with a value of <a:math xmlns:a="http://www.w3.org/1998/Math/MathML" id="M1"> <a:mi>p</a:mi> <a:mo>=</a:mo> <a:mn>0.001</a:mn> </a:math> <c:math xmlns:c="http://www.w3.org/1998/Math/MathML" id="M2"> <c:mfenced open="(" close=")" separators="|"> <c:mrow> <c:mi>p</c:mi> <c:mo><</c:mo> <c:mn>0.05</c:mn> </c:mrow> </c:mfenced> </c:math> , resulting in a significant difference in wound size reduction between the groups. Further post hoc analysis using the Mann–Whitney test indicated a significant difference between the control groups and the treatment groups (P0, P1 versus P3, P4, P5, P8, P9, and P10) with a value of <h:math xmlns:h="http://www.w3.org/1998/Math/MathML" id="M3"> <h:mi>p</h:mi> <h:mo>=</h:mo> <h:mn>0.009</h:mn> </h:math> <j:math xmlns:j="http://www.w3.org/1998/Math/MathML" id="M4"> <j:mfenced open="(" close=")" separators="|"> <j:mrow> <j:mi>p</j:mi> <j:mo><</j:mo> <j:mn>0.05</j:mn> </j:mrow> </j:mfenced> </j:math> . Conclusions. Ozonated Aloe vera oil is effective in increasing the healing response of full-thickness defects, leading to the increase in the number of fibroblasts and collagen thickening that in turn accelerates wound healing in Sprague-Dawley rats.