Rapid and sensitive point-of-care PTS-CRISPR assay for food safety monitoring of aflatoxin B1
Ziqiang Deng, Jin Zhou, Chaoqun Wang, Jianyu Hu, Rui Liu, Yi Lv
Abstract
Food safety is crucial for human health, but its effective on-site monitoring remains a challenge. Pregnancy test strip (PTS) is the successful point-of-care testing (POCT) product of the highest market share in the world, with the cost as low as $0.10 per test. Herein, combined with the CRISPR (clustered regularly interspaced short palindromic repeats) system, PTS-CRISPR was for the first time introduced into food safety monitoring, for rapid and sensitive POCT of aflatoxin B1. The low-cost, easy-to-operate PTS-CRISPR is expected to bring security to the grassroots food market.
Topics & Concepts
AflatoxinCRISPRFood safetyPoint of careFood scienceChemistryBiotechnologyToxicologyBiologyMedicineBiochemistryGeneNursingBiosensors and Analytical DetectionListeria monocytogenes in Food SafetyIdentification and Quantification in Food