Label-Free and Template-Free Chemiluminescent Biosensor for Sensitive Detection of 5-Hydroxymethylcytosine in Genomic DNA
Chenchen Li, Yue-hong Dong, Xiaoran Zou, Xiliang Luo, Dazhong Shen, Juan Hu, Chun‐yang Zhang
Abstract
ng/μL. Moreover, this method can distinguish 5hmC from 5-methylcytosine (5mC) and cytosine (C) and even discriminate 0.1% 5hmC in the mixture of 5hmC-DNA and 5mC-DNA. Importantly, this hmC-GLIB-IAS strategy enables genome-wide analysis without the involvement of either isotope-labeled substrates or specific antibodies, providing a powerful platform to detect 5hmC in real genomic DNA with high reproducibility and accuracy.
Topics & Concepts
Chemistry5-HydroxymethylcytosineChemiluminescenceBiosensorDNAgenomic DNAComputational biologyDetection limitChromatographyBiochemistryGeneDNA methylationGene expressionBiologyAmino Acid Enzymes and MetabolismEpigenetics and DNA MethylationPolyamine Metabolism and Applications