Proteomic Study of the Interactions between Phages and the Bacterial Host <i>Klebsiella pneumoniae</i>
Inés Bleriot, Lucía Blasco, Olga Pacios, Laura Fernández‐García, María López, Concha Ortiz-Cartagena, Antonio Barrio-Pujante, Felipe Fernández-Cuenca, Álvaro Pascual, Luis Martı́nez-Martı́nez, Jesús Oteo, María Tomás
Abstract
. Viral defense mechanisms included restriction-modification system evasion, the toxin-antitoxin (TA) system, DNA degradation evasion, blocking of host restriction and modification, and resistance to the abortive infection system, anti-CRISPR and CRISPR-Cas systems. Regarding bacterial defense mechanisms, proteomic analysis revealed expression of proteins involved in the prophage (FtsH protease modulator), plasmid (cupin phosphomannose isomerase protein), defense/virulence/resistance (porins, efflux pumps, lipopolysaccharide, pilus elements, quorum network proteins, TA systems, and methyltransferases), oxidative stress mechanisms, and Acr candidates (anti-CRISPR protein). The findings reveal some important molecular mechanisms involved in the phage-host bacterial interactions; however, further study in this field is required to improve the efficacy of phage therapy.