Improvement of (<i>S</i>)-selective imine reductase GF3546 for the synthesis of chiral cyclic amines
Yuta Fukawa, Keita YOSHIDA, Sayaka Degura, Koichi Mitsukura, Toyokazu Yoshida
Abstract
sp. GF3546 (SIR46) showed twice the activity of the wild type and high thermostability at 50 °C. Using a biocatalyst based on SIR46, we investigated the activity toward high-concentration 2-methyl-1-pyrroline up to 500 mM, conversion rates of other imines, and reductive amination activity.
Topics & Concepts
ImineChemistryCombinatorial chemistryStereochemistryOrganic chemistryCatalysisEnzyme Catalysis and ImmobilizationBiochemical and Molecular ResearchEnzyme Structure and Function