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Transcriptional profiling reveals signatures of latent developmental potential in <i>Arabidopsis</i> stomatal lineage ground cells

Chin‐Min Kimmy Ho, Martin Bringmann, Yoshimi Oshima, Nobutaka Mitsuda, Dominique C. Bergmann

2021Proceedings of the National Academy of Sciences34 citationsDOIOpen Access PDF

Abstract

leaf epidermis as a model to explore how cells might balance potential to differentiate with a reentry into proliferation. By generating transcriptomes of fluorescence-activated cell sorting-isolated populations that combinatorically define SLGCs and integrating these data with other stomatal lineage datasets, we find that SLGCs appear poised between proliferation and endoreduplication. Furthermore, we found the RNA polymerase II-related mediator complex interactor DEK and the transcription factor MYB16 accumulate differentially in the stomatal lineage and influence the extent of cell proliferation during leaf development. These findings suggest that SLGC latent potential is maintained by poising of the cell cycle machinery, as well as general and site-specific gene-expression regulators.

Topics & Concepts

ArabidopsisBiologyLineage (genetic)Profiling (computer programming)Evolutionary biologyCell biologyGeneGeneticsComputer scienceOperating systemMutantPlant Molecular Biology ResearchPlant nutrient uptake and metabolismLight effects on plants