Fluorene-9-bisphenol regulates steroidogenic hormone synthesis in H295R cells through the AC/cAMP/PKA signaling pathway
Yuan Huang, Wei Zhang, Na Cui, Zhiming Xiao, Wenyu Zhao, Ruiguo Wang, John P. Giesy, Xiaoou Su
Abstract
by down-regulation of steroidogenic genes. Inhibition of AC activity, intercellular cAMP content and PKA activity after exposure to BHPF implied that the AC/cAMP/PKA signaling pathway was involved in BHPF-induced suppression of steroidogenesis in H295R cells. Additionally, BHPF inhibited steroidogenesis and expressions of steroidogenic genes via decreasing expression of SF-1 protein, both in basal and dbcAMP-induced treatment. These results contributed to understanding molecular mechanisms of BHPF-induced effects on steroidogenesis and advancing the comprehensive risk assessment of BPs.
Topics & Concepts
Steroidogenic acute regulatory proteinCholesterol side-chain cleavage enzymeEndocrinologyInternal medicineAldosteroneAdrenocortical carcinomaSteroid 11-beta-hydroxylaseChemistryAdenylate kinaseHormonecAMP-dependent pathwayForskolinCyclaseBiologySteroidAdenylyl cyclaseGene expressionCytochrome P450BiochemistryReceptorGeneMedicineMetabolismStimulationEffects and risks of endocrine disrupting chemicalsEstrogen and related hormone effectsVitamin D Research Studies