Molecular Diagnosis of Leishmaniasis in Spain: Development and Validation of Ready-To-Use Gel-Form Nested and Real-Time PCRs To Detect <i>Leishmania</i> spp.
Carmen Chicharro, Javier Nieto, Silvia Migueláñez, E. Barbero García, Sheila Ortega, Ana Peña, José Miguel Rubio, María Flóres-Chávez
Abstract
spp. These new approaches could be introduced even in middle- and low-resource laboratories; one is a ready-to-use gel-form system of a nested PCR and the other is a real-time PCR. We show why molecular diagnosis is the best methodology to confirm a clinical suspicion of leishmaniasis with higher sensitivity than traditional methods, thus facilitating early diagnosis and timely treatment of human leishmaniasis.
Topics & Concepts
Visceral leishmaniasisLeishmania infantumLeishmaniasisSerologyGold standard (test)Nested polymerase chain reactionCanine leishmaniasisPolymerase chain reactionZoonosisLeishmaniaBiologyVirologyParasite hostingVeterinary medicineMedicineImmunologyGeneGeneticsInternal medicineAntibodyComputer scienceWorld Wide WebResearch on Leishmaniasis Studies