Development of an immunocolloidal strip for rapid detection of picoxystrobin
Shanshan Song, Ziwen Chen, Jie Liu, Liguang Xu, Hua Kuang, Jianping Zhu, Liqiang Liu
Abstract
In this study, we developed a monoclonal antibody against picoxystrobin (PIC). After cell fusion, four cell lines (3C2, 4D1, 4G4 and 5D4) capable of producing monoclonal antibodies against oxystrobin were obtained. The monoclonal antibody produced by the cell line 3C2 was the most sensitive to PIC, and its IC50 value was 0.1 ng/ml. The monoclonal antibody was then used to prepare immunochromatographic strips, and its cut-off values in both phosphate-buffered saline solution and cucumber samples were 50 and 100 ng/ml, respectively. It was concluded that the ICS can sensitively and rapidly detect PIC in cucumber samples.
Topics & Concepts
Monoclonal antibodyChemistryCell fusionPhosphate buffered salineChromatographyMolecular biologyMonoclonalAntibodyCellBiologyBiochemistryImmunologyPharmacological Effects and AssaysMass Spectrometry Techniques and ApplicationsAnalytical Chemistry and Sensors