Red cell proteasome modulation by storage, redox metabolism and transfusion.
Vassilis L. Tzounakas, Monika Dzieciątkowska, Alkmini T. Anastasiadi, Dimitrios G. Karadimas, Athina Vergaki, Panagiotis Siourounis, Konstantinos Stamoulis, Issidora S. Papassideri, Anastasios G. Kriebardis, Angelo D’Alessandro, Marianna H. Antonelou
Abstract
BACKGROUND: ) on the activity and topology of red blood cell (RBC) proteasomes. MATERIALS AND METHODS: donors following storage in leukoreduced units. RBC proteasome activity was also tested in transfusion-mimicking conditions in vitro. RESULTS: metabolome and quality markers of the RBC units. The storage-induced modifications in the proteasome activities were only partly restored in transfusion-mimicking conditions. DISCUSSION: Storage conditions and G6PD deficiency affect (individually and in synergy) the abundance, distribution, activity, and release of RBC proteasomes. The partial irreversibility of these effects in transfusion-mimicking conditions demands further investigation of their clinical impact on transfusion outcomes.