Caveolin-1 forms a complex with P2X7 receptor and tunes P2X7-mediated ATP signaling in mouse bone marrow-derived macrophages
Yuuki Sawai, Yoshiaki Suzuki, Masataka Asagiri, Shigeaki Hida, Rubii Kondo, Gerald W. Zamponi, Wayne R. Giles, Yuji Imaizumi, Hisao Yamamura
Abstract
In bone marrow-derived macrophages, Cav-1 suppresses the macropore formation of P2X7 receptors through their direct or indirect interactions, resulting in reduced membrane permeability of cations (Ca 2+ and K + ) and large cell-impermeable dye (TO-PRO3) induced by ATP. Cav-1 also inhibits ATP-induced IL-1β secretion, ROS production, GSDMD cleavage, and pyroptosis. Cav-1 contributes to the maintenance of proper immune responses by finely tuning IL-1β secretion and cell death in macrophages.
Topics & Concepts
PyroptosisReceptorPurinergic receptorCell biologyCytosolProinflammatory cytokineExtracellularChemistryBiologyInflammasomeInflammationBiochemistryImmunologyEnzymeAdenosine and Purinergic Signaling