The Association Between Fecal Short-Chain Fatty Acids, Gut Microbiota, and Visceral Fat in Monozygotic Twin Pairs
Xing-Qi Yin, Ya-Xin An, Cai-Guo Yu, Jing Ke, Dong Zhao, Ke Yu
Abstract
Purpose: To examine the association of short-chain fatty acids (SCFAs), gut microbiota and obesity in individual twins and to control for genetic and shared environmental effects by studying monozygotic intrapair differences. Patients and Methods: The study recruited 20 pairs of monozygotic twins. Body composition measurements were performed by using the multi-frequency bioelectrical impedance technique. SCFAs were extracted from feces and quantified by gas chromatography-mass spectrometer. Gut microbiota was evaluated by 16S rRNA gene sequencing. Results: Fecal SCFAs were negatively correlated with adiposity parameters including body mass index, visceral adipose tissue and waist circumference (all P < 0.05). Metastat analysis showed that the top 5 relatively abundant bacterial taxa of viscerally obese and non-obese groups were Bacteroides, Collinsella, Eubacterium rectale group, Lachnoclostridium , and Tyzzerella . Participants with visceral obesity had lower abundance of Bacteroides and Collinsella compared to non-obese patients ( P < 0.05). Among them, the abundance of Collinsella was positively correlated with acetic acid concentrations ( r = 0.63, P = 0.011). There were no significant intrapair differences in each SCFA concentrations between the twins in our study ( P > 0.05). Conclusion: Low fecal concentrations of SCFAs were associated with visceral obesity, and the gut microbiota might be involved in the underlying mechanism. Keywords: twins, short-chain fatty acids, gut microbiota, obesity, visceral fat