Base-Resolution Analysis of Deoxyuridine at the Genome Scale Based on the Artificial Incorporation Modified Nucleobase
Yafen Wang, Xiong Zhang, Shaoqing Han, Wei Yang, Zonggui Chen, Fan Wu, Jizhou Liu, Xiaocheng Weng, Xiang Zhou
Abstract
Deamination of cytosine and dUMP misincorporation have been found to be capable of producing uracil in the genome. This study presents the AI-seq (artificial incorporation modified nucleobase for sequencing), a "base substitution", which not only is capable of profiling uracil at single-nucleotide resolution and showing its centromeric enrichment but could also reveal that the identified uracil sites are derived from cytosine deamination. All the results indicate the potential biological significance of uracil as the epigenetic modification.
Topics & Concepts
DeaminationNucleobaseUracilCytosineGenomeNucleotideDeoxyuridineChemistryDNABiologyGeneticsComputational biologyBiochemistryGeneEnzymeRNA modifications and cancerEpigenetics and DNA MethylationRNA and protein synthesis mechanisms