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Synthesis, in vitro urease inhibitory potential and molecular docking study of benzofuran-based-thiazoldinone analogues

Muhammad Taha, Fazal Rahim, Hayat Ullah, Abdul Wadood, Rai Khalid Farooq, Syed Adnan Alı Shah, Muhammad Nawaz, Zainul Amiruddin Zakaria

2020Scientific Reports41 citationsDOIOpen Access PDF

Abstract

Abstract In continuation of our work on enzyme inhibition, the benzofuran-based-thiazoldinone analogues ( 1–14 ) were synthesized, characterized by HREI-MS, 1 H and 13 CNMR and evaluated for urease inhibition. Compounds 1–14 exhibited a varying degree of urease inhibitory activity with IC 50 values between 1.2 ± 0.01 to 23.50 ± 0.70 µM when compared with standard drug thiourea having IC 50 value 21.40 ± 0.21 µM. Compound 1, 3, 5 and 8 showed significant inhibitory effects with IC 50 values 1.2 ± 0.01, 2.20 ± 0.01, 1.40 ± 0.01 and 2.90 ± 0.01 µM respectively, better than the rest of the series. A structure activity relationship (SAR) of this series has been established based on electronic effects and position of different substituents present on phenyl ring. Molecular docking studies were performed to understand the binding interaction of the compounds.

Topics & Concepts

BenzofuranUreaseThioureaChemistryDocking (animal)StereochemistryIn vitroEnzymeLead compoundActive siteInhibitory postsynaptic potentialBiochemistryOrganic chemistryBiologyMedicineNeuroscienceNursingSynthesis and biological activityMicrobial Applications in Construction MaterialsSynthesis and Characterization of Heterocyclic Compounds
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