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<i>Bioconductor</i> toolchain for reproducible bioinformatics pipelines using <i>Rcwl</i> and <i>RcwlPipelines</i>

Qiang Hu, Alan D. Hutson, Song Liu, Martin Morgan, Qian Liu

2021Bioinformatics13 citationsDOIOpen Access PDF

Abstract

SUMMARY: The Common Workflow Language (CWL) is used to provide portable and reproducible data analysis workflows across different tools and computing environments. We have developed Rcwl, an R interface to CWL, to provide easier development, use and maintenance of CWL pipelines from within R. We have also collected more than 100 pre-built tools and pipelines in RcwlPipelines, ready to be queried and used by researchers in their own analysis. A single-cell RNA sequencing preprocessing pipeline demonstrates use of the software. AVAILABILITY AND IMPLEMENTATION: Project website: https://rcwl.org (Rcwl: https://bioconductor.org/packages/Rcwl; RcwlPipelines: https://bioconductor.org/packages/RcwlPipelines). SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.

Topics & Concepts

BioconductorToolchainWorkflowComputer sciencePipeline (software)PreprocessorSoftwareSoftware engineeringWorkbenchPipeline transportInterface (matter)Programming languageData miningDatabaseOperating systemVisualizationEngineeringBiologyBubbleEnvironmental engineeringBiochemistryGeneMaximum bubble pressure methodSingle-cell and spatial transcriptomicsCell Image Analysis TechniquesScientific Computing and Data Management
<i>Bioconductor</i> toolchain for reproducible bioinformatics pipelines using <i>Rcwl</i> and <i>RcwlPipelines</i> | Litcius