Secondary modification of oxidatively-modified proline N-termini for the construction of complex bioconjugates
Johnathan C. Maza, Alexandra V. Ramsey, Meire Mehare, Shane W. Krska, Craig A. Parish, Matthew B. Francis
Abstract
A convenient two-step method is reported for the ligation of alkoxyamine- or hydrazine-bearing cargo to proline N-termini. Using this approach, bifunctional proline N-terminal bioconjugates are constructed and proline N-terminal proteins are immobilized.
Topics & Concepts
BifunctionalChemistryOximeHydrazoneCombinatorial chemistryPosttranslational modificationStereochemistryBiochemistryEnzymeCatalysisClick Chemistry and ApplicationsChemical Synthesis and AnalysisPeptidase Inhibition and Analysis