Kinetics of Toehold-Mediated DNA Strand Displacement Depend on Fe<sup>II</sup><sub>4</sub>L<sub>4</sub> Tetrahedron Concentration
Jinbo Zhu, Filip Bošković, Bao‐Nguyen T. Nguyen, Jonathan R. Nitschke, Ulrich F. Keyser
Abstract
binding to DNA. SDRs mediated by toehold ends in different lengths (3-5) were investigated as a function of cage concentration. Their reaction rates all first increased and then decreased as cage concentration increased. We infer that cage binding on the toehold end slows SDR, whereas the stabilization of intermediates that contain two overhangs accelerates SDR. The tetrahedral cage thus serves as a versatile tool for modulation of SDR kinetics.
Topics & Concepts
TetrahedronKineticsDNACageChemistryDNA nanotechnologyBiophysicsNanotechnologyCrystallographyMaterials sciencePhysicsBiologyBiochemistryMathematicsCombinatoricsQuantum mechanicsAdvanced biosensing and bioanalysis techniquesRNA Interference and Gene DeliveryDNA and Nucleic Acid Chemistry