Generation of pancreatic progenitors from human pluripotent stem cells by small molecules
Yuqian Jiang, Chuanxin Chen, Lauren N. Randolph, Songtao Ye, Xin Zhang, Xiaoping Bao, Xiaojun Lian
Abstract
DE cells. BMP inhibition further enhances this DE differentiation, generating over 87% DE cells. These DE cells could be further differentiated into PPs and functional β cells. RNA-sequencing analysis of PP differentiation from hPSCs revealed expected transcriptome dynamics and new gene regulators during our small-molecule PP differentiation protocol. Overall, we established a robust growth-factor-free protocol for generating DE and PP cells, facilitating scalable production of pancreatic cells for regenerative applications.
Topics & Concepts
BiologyWnt signaling pathwayCell biologyDirected differentiationInduced pluripotent stem cellCellular differentiationProgenitor cellStem cellTranscriptomeFOXA2Embryonic stem cellSignal transductionGene expressionGeneticsGenePancreatic function and diabetesPluripotent Stem Cells ResearchRenal and related cancers