A single-cell multi-omic atlas spanning the adult rhesus macaque brain
Kenneth L. Chiou, Xingfan Huang, Martin O. Bohlen, Sébastien Tremblay, Alex R. DeCasien, Diana R. O’Day, Cailyn H. Spurrell, Aishwarya A. Gogate, Trisha M. Zintel, Madeline G. Andrews, Melween I. Martínez, Lea M. Starita, Michael J. Montague, Michael L. Platt, Jay Shendure, Noah Snyder‐Mackler
Abstract
Cataloging the diverse cellular architecture of the primate brain is crucial for understanding cognition, behavior, and disease in humans. Here, we generated a brain-wide single-cell multimodal molecular atlas of the rhesus macaque brain. Together, we profiled 2.58 M transcriptomes and 1.59 M epigenomes from single nuclei sampled from 30 regions across the adult brain. Cell composition differed extensively across the brain, revealing cellular signatures of region-specific functions. We also identified 1.19 M candidate regulatory elements, many previously unidentified, allowing us to explore the landscape of cis-regulatory grammar and neurological disease risk in a cell type-specific manner. Altogether, this multi-omic atlas provides an open resource for investigating the evolution of the human brain and identifying novel targets for disease interventions.