Pathogen Detection in Infective Endocarditis Using Targeted Metagenomics on Whole Blood and Plasma: a Prospective Pilot Study
Laure Flurin, Matthew J. Wolf, Cody R. Fisher, Edison Cano Cevallos, James J. Vaillant, Bobbi S. Pritt, Daniel C. DeSimone, Robin Patel
Abstract
= 0.47). When results of whole blood and plasma testing were combined, a positive tMGS result was found in 23 subjects (66%). tMGS identified a potential pathogen in 5 of 6 culture-negative IE cases. Although further study is needed, the results of this pilot study suggest that blood-based tMGS may provide pathogen identification in subjects with IE, including in culture-negative cases.
Topics & Concepts
MetagenomicsInfective endocarditisPathogenMicrobiologyEndocarditisBlood cultureBacteremiaBiologyProspective cohort studyVirologyMedicineInternal medicineAntibioticsGeneBiochemistryInfective Endocarditis Diagnosis and ManagementBacterial Identification and Susceptibility TestingAntimicrobial Resistance in Staphylococcus