Microbial degradation of carbamazepine by a newly isolated of Gordonia polyophrenivorans
Yun Wang, Jianfeng Gao, Zhou Suo, Mengjia Lian
Abstract
The bacterial degradation of carbamazepine (CBZ) is an effective way not to produce toxic and harmful substances, which provides a new possibility for environmental remediation. In this study, a bacterium that can degrade carbamazepine was isolated from wastewater. The 16S rDNA gene cluster analysis identified the strain as Gordonia polyophrenivorans C. Its degradation rate was determined by high-performance liquid chromatography (HPLC), and intermediate products were determined by gas chromatography/mass spectrometry (GC). The dibenzepine and dibenzazepine-10, 11-dione metabolites were found in the bacteria. Its complete genome was 5,925,347 bp in length and encoded 5300 genes. The whole genome sequencing results confirmed that the strain had gene fragments encoding amide hydrolase, double oxygenase, fructose-bisphosphate aldolase and lyase. The expression and significance of amide hydrolase in the strain were elucidated. The expression of amide hydrolase in this strain and its significance were clarified. The carbamazepine degradation pathway in bacteria was reported for the first time.