Optimization of media composition for enhancing tetracycline degradation by <i>Trichosporon mycotoxinivorans</i> XPY-10 using response surface methodology
Xiaochen Huang, Xinyang Zhang, Yanyan Huang, Xuping Xu
Abstract
The objective of the study was to improve tetracycline degradation efficiency by Trichosporon mycotoxinivorans XPY-10 using statistical experimental designs. Different culture conditions (FeSO4, pH and glucose) were optimized for tetracycline biodegradation and the mutual interactions between these three variables were analysed using Box–Behnken design (BBD) and response surface methodology (RSM). The results showed that the empirical model was suitable for experimental data, and the maximum tetracycline degradation efficiency by XPY-10 was 95.18% under the optimum conditions of 0.02% of FeSO4, pH 7.83 and 0.28% of glucose, which was further verified by experiments. This study indicated the excellent ability of XPY-10 in degrading tetracycline and theoretical support for the follow-up practice to remediate tetracycline contaminated environment.