Engineering the Outer Membrane Could Facilitate Better Bacterial Performance and Effectively Enhance Poly-3-Hydroxybutyrate Accumulation
Jianli Wang, Wenjian Ma, Yu Fang, Hailing Zhang, Hao Liang, Haili Liu, Tingwei Wang, Shangwei Chen, Jian Ji, Xiaoyuan Wang
Abstract
Understanding the detailed influence of the OM on the cell envelope and cellular metabolism is important for optimizing the E. coli cell factory and many other microorganisms. This study revealed the applicability of remodeling the OM to enhance PHB accumulation as representative inclusion bodies. The results generated in this study give essential information for producing other inclusion bodies or chemicals which need more acetyl-CoA and cofactors but less by-product acids. This study is promising to provide new ideas for the improvement of microbial cell factories.
Topics & Concepts
Bacterial outer membraneEscherichia coliChemistryFimbriaCell envelopePolysaccharideCellBiochemistryFermentationBacteriaCell biologyHeterologousLipopolysaccharideMembranePolyhydroxyalkanoatesCell membraneBiophysicsCell wallStrain (injury)Metabolic engineeringBacterial cell structureBiosynthesisNucleoidSecretionEnterobacteriaceaeMetabolismBiologyCell cultureFlux (metallurgy)Fatty acidIntracellularbiodegradable polymer synthesis and propertiesMicrobial Metabolic Engineering and BioproductionBacterial Genetics and Biotechnology