Seroprevalence and Associated Risk Factors of Leptospira interrogans Serogroup Sejroe Serovar Hardjo in Dairy Farms in and around Jimma Town, Southwestern Ethiopia
Garoma Desa, Yosef Deneke, Feyissa Begna, Tadele Tolosa
Abstract
A cross-sectional study was conducted on selected dairy farms in and around Jimma town, Oromia, southwestern Ethiopia from November 2019 to May 2020 to determine the seroprevalence of Leptospira interrogans serogroup Sejroe serovar Hardjo (L. hardjo). Furthermore, information was gathered on individual animal and herd level by using pretested semistructured questionnaire to assess associated risk factors. A stratified and simple random sampling procedure was used for the selection of dairy farms and individual animal’s, respectively. Indirect enzyme-linked immunosorbent assay (I-ELISA) was used in this study to detect antibody against L. hardjo. Out of 384 animal’s sera, 94 animals were seropositive against L. hardjo antibodies. From 77 dairy farms selected for the study, 57 of them were distinguished as positive for L. hardjo. The overall seroprevalence of leptospirosis caused by L. hardjo was 24.48% (95% CI: 20.18%–28.78%) and 74.03% (95% CI: 64.23%–83.82%) at individual animal and farm level, respectively. The result of multilogistic regression analysis revealed that management system ( <a:math xmlns:a="http://www.w3.org/1998/Math/MathML" id="M1"><a:mi>p</a:mi></a:math> < 0.05; OR = 4.25 (95% CI: 2.31–7.82)), hygienic status of the farm ( <c:math xmlns:c="http://www.w3.org/1998/Math/MathML" id="M2"><c:mi>p</c:mi></c:math> < 0.05; OR = 0.35 (95% CI: 0.20–0.61)), age of animals ( <e:math xmlns:e="http://www.w3.org/1998/Math/MathML" id="M3"><e:mi>p</e:mi></e:math> < 0.05; OR = 8.30 (95% CI: 1.87–36.89)), history of abortion ( <g:math xmlns:g="http://www.w3.org/1998/Math/MathML" id="M4"><g:mi>p</g:mi></g:math> < 0.05; OR = 8.37 (95% CI: 1.73–40.42)), herd size ( <i:math xmlns:i="http://www.w3.org/1998/Math/MathML" id="M5"><i:mi>p</i:mi></i:math> < 0.05; OR = 2.32 (95% CI: 1.17–4.61)), and access of rodents to the farm ( <k:math xmlns:k="http://www.w3.org/1998/Math/MathML" id="M6"><k:mi>p</k:mi></k:math> < 0.05; OR = 0.17 (95% CI: 0.03–0.86)) were significantly associated with the occurrence of L. hardjo infection. However, breed, parity, and introduction of new animals to the farm were insignificantly associated ( <m:math xmlns:m="http://www.w3.org/1998/Math/MathML" id="M7"><m:mi>p</m:mi></m:math> > 0.05). Management system of the animal, hygienic status of the farm, herd size, age of animals, previous history of abortion, and access of rodents to the farm were identified as potential risk factors of L. hardjo disease occurrence. Thus, limiting rodents contact with cattle and their feed and water as well as good sanitary practices and husbandry management should be undertaken.