Arf6-mediated macropinocytosis-enhanced suicide gene therapy of C16TAB-condensed Tat/pDNA nanoparticles in ovarian cancer
Zhe Sun, Jinhai Huang, Linjia Su, Jing Li, Fangzheng Qi, Huishan Su, Yanan Chen, Qing Zhang, Qiangzhe Zhang, Zongjin Li, Sihe Zhang
Abstract
molecular imaging based on DF (Fluc-eGFP)-TF (RFP-Rluc-HSV-ttk) system showed that T-P-C nanoparticles significantly increased the targeted delivery and suicide gene therapy in a mouse model xenografted with human ovarian cancer. More importantly, Arf6-mediated macropinocytosis remarkably enhanced the delivery efficiency and suicide gene therapy effect of T-P-C nanoparticles. Therefore, these C16TAB-condensed Tat/pDNA nanoparticles combined with the dual molecular imaging strategy provides a novel intracellular delivery platform for high-efficient, precise suicide gene therapy of ovarian cancer.
Topics & Concepts
PinocytosisNanoparticleOvarian cancerCancerChemistryMedicineNanotechnologyMaterials scienceEndocytosisBiochemistryInternal medicineCellRNA Interference and Gene DeliveryVirus-based gene therapy researchAdvanced biosensing and bioanalysis techniques