Pt(II) versus Pt(IV) in Carbene Glycoconjugate Antitumor Agents: Minimal Structural Variations and Great Performance Changes
Alfonso Annunziata, Angela Amoresano, Maria Elena Cucciolito, Roberto Esposito, Giarita Ferraro, Ilaria Iacobucci, Paola Imbimbo, Rosanna Lucignano, Massimo Melchiorre, Maria Monti, Chiara Scognamiglio, Angela Tuzi, Daria Maria Monti, Antonello Merlino, Francesco Ruffo
Abstract
were evaluated on the same panel of cell lines. DNA and protein binding properties were assessed using human serum albumin, the model protein hen egg white lysozyme, and double stranded DNA model systems by a variety of experimental techniques, including UV-vis absorption spectroscopy, fluorescence, circular dichroism, and electrospray ionization mass spectrometry. Although the compounds present similar structures, their in-solution stability, cellular uptake, and DNA binding properties are diverse. These differences may represent the basis of their different cytotoxicity and biological activity.