Japanese Encephalitis Virus NS4A Protein Interacts with PTEN-Induced Kinase 1 (PINK1) and Promotes Mitophagy in Infected Cells
Anshu Agarwal, Mohd Faraz Alam, Brohmomoy Basu, S Pattanayak, Shailendra Asthana, Gulam Hussain Syed, Manjula Kalia, Sudhanshu Vrati
Abstract
The JEV-infected mammalian cells show an enhanced mitophagy flux with a concomitant decline in the mitochondrial mass. We show that the NS4A protein of JEV localized to the mitochondria and interacted with PINK1 in Huh7 cells during infection with the virus and demonstrate that JEV-NS4A alone is sufficient to induce mitophagy. The study provides the first evidence of mitochondrial quality control dysregulation during JEV infection, largely mediated by its NS4A protein.
Topics & Concepts
MitophagyPINK1AutophagyVirusVirologyPTENBiologyCell biologyMitochondrionKinaseParkinApoptosisSignal transductionPI3K/AKT/mTOR pathwayMedicineGeneticsParkinson's diseaseDiseaseInternal medicineMosquito-borne diseases and controlAutophagy in Disease and TherapyCytomegalovirus and herpesvirus research