Acoustic hologram–induced virtual in vivo enhanced waveguide (AH-VIEW)
Qibo Lin, Zhaoxi Li, Bo Wang, Mengqing Zhou, Yang Xie, Danfeng Wang, Chenxue Hou, Runyu Wang, Xiangdong Liu, Xin Sun, Shan Han, Ziyan Chen, Ziyan Chen, Huayi Wu, Yintang Yang, Chunlong Fei, Zeyu Chen, Zeyu Chen
Abstract
Optical imaging and phototherapy in deep tissues face notable challenges due to light scattering. We use encoded acoustic holograms to generate three-dimensional acoustic fields within the target medium, enabling instantaneous and robust modulation of the volumetric refractive index, thereby noninvasively controlling the trajectory of light. Through this approach, we achieved a remarkable 24.3% increase in tissue heating rate in vitro photothermal effect tests on porcine skin. In vivo photoacoustic imaging of mouse brain vasculature exhibits an improved signal-to-noise ratio through the intact scalp and skull. These findings demonstrate that our strategy can effectively suppress light scattering in complex biological tissues by inducing low-angle scattering, achieving an effective depth reaching the millimeter scale. The versatility of this strategy extends its potential applications to neuroscience, lithography, and additive manufacturing.