Towards superior mRNA caps accessible by click chemistry: synthesis and translational properties of triazole-bearing oligonucleotide cap analogs
Mateusz Kozarski, Karolina Drążkowska, Marcelina Bednarczyk, Marcin Warmiński, Jacek Jemielity, Joanna Kowalska
Abstract
mRNA-based gene delivery is a powerful strategy for many therapeutic areas. In this work, we used CuAAC to synthesize next-generation triazole-bearing mRNA 5' cap analogs and evaluated them as reagents for modification of in vitro transcribed mRNA.
Topics & Concepts
Click chemistryOligonucleotidePhosphodiester bondMessenger RNAChemistryT7 RNA polymeraseRNATranslational efficiencyTranslation (biology)RibozymeProtein biosynthesisTriazoleBiochemistryCombinatorial chemistryDNAGeneBacteriophageEscherichia coliOrganic chemistryRNA Interference and Gene DeliveryVirus-based gene therapy researchAdvanced biosensing and bioanalysis techniques